Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars.
Identifieur interne : 000134 ( Main/Exploration ); précédent : 000133; suivant : 000135Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars.
Auteurs : Samia Daldoul [Allemagne] ; Sabine Guillaumie ; Götz M. Reustle ; Gabi Krczal ; Abdelwahed Ghorbel ; Serge Delrot ; Ahmed Mliki ; Michael U. HöferSource :
- Plant science : an international journal of experimental plant biology [ 1873-2259 ] ; 2010.
Abstract
Salt stress adversely affects the growth of grapevine plants. In order to understand the molecular basis of salt stress response in grapevine plants, suppression subtractive hybridization (SSH) and microarray based screening approaches were combined. Two leaf-specific subtractive cDNA libraries were constructed from grapevine plants subjected to a moderate, incremental salt stress treatment. SSH were performed 6h and 24h after NaCl peaked at 100mM using cDNAs prepared from leaves of a salt tolerant cultivar (Razegui) as testers and cDNAs from unstressed leaves as drivers. Then, a pre-screened subset of cDNA clones from these SSH libraries were used to construct a Vitis vinifera cDNA array, in order to verify the expression changes of the genes upon salt treatment. Expression profiles were compared between the salt tolerant and a susceptible cultivar (Syrah) under both control conditions and after salt stress treatment. Seven cDNA clones were identified which were up-regulated by salt stress in two independent growth experiments and confirmed by RNA blot analysis. The transcript expression patterns of the selected genes differed between the contrasting grapevine cultivars tested with respect to stress-regulation. The possible relationship of individual cDNAs with salinity tolerance mechanisms is discussed.
DOI: 10.1016/j.plantsci.2010.07.017
PubMed: 21802607
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000688
- to stream PubMed, to step Curation: 000684
- to stream PubMed, to step Checkpoint: 000712
- to stream Main, to step Merge: 000134
- to stream Main, to step Curation: 000134
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars.</title>
<author><name sortKey="Daldoul, Samia" sort="Daldoul, Samia" uniqKey="Daldoul S" first="Samia" last="Daldoul">Samia Daldoul</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Biotechnologie de Borj cédria, Laboratoire de Physiologie Moléculaire des Plantes, B.P.901, 2050 Hammam-Lif, Tunisia; RLP-Agroscience GmbH/Alplanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt and der Weinstraße, Germany. samiabiotech@yahoo.fr</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Centre de Biotechnologie de Borj cédria, Laboratoire de Physiologie Moléculaire des Plantes, B.P.901, 2050 Hammam-Lif, Tunisia; RLP-Agroscience GmbH/Alplanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt and der Weinstraße</wicri:regionArea>
<wicri:noRegion>67435 Neustadt and der Weinstraße</wicri:noRegion>
<wicri:noRegion>67435 Neustadt and der Weinstraße</wicri:noRegion>
<wicri:noRegion>67435 Neustadt and der Weinstraße</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guillaumie, Sabine" sort="Guillaumie, Sabine" uniqKey="Guillaumie S" first="Sabine" last="Guillaumie">Sabine Guillaumie</name>
</author>
<author><name sortKey="Reustle, Gotz M" sort="Reustle, Gotz M" uniqKey="Reustle G" first="Götz M" last="Reustle">Götz M. Reustle</name>
</author>
<author><name sortKey="Krczal, Gabi" sort="Krczal, Gabi" uniqKey="Krczal G" first="Gabi" last="Krczal">Gabi Krczal</name>
</author>
<author><name sortKey="Ghorbel, Abdelwahed" sort="Ghorbel, Abdelwahed" uniqKey="Ghorbel A" first="Abdelwahed" last="Ghorbel">Abdelwahed Ghorbel</name>
</author>
<author><name sortKey="Delrot, Serge" sort="Delrot, Serge" uniqKey="Delrot S" first="Serge" last="Delrot">Serge Delrot</name>
</author>
<author><name sortKey="Mliki, Ahmed" sort="Mliki, Ahmed" uniqKey="Mliki A" first="Ahmed" last="Mliki">Ahmed Mliki</name>
</author>
<author><name sortKey="Hofer, Michael U" sort="Hofer, Michael U" uniqKey="Hofer M" first="Michael U" last="Höfer">Michael U. Höfer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:21802607</idno>
<idno type="pmid">21802607</idno>
<idno type="doi">10.1016/j.plantsci.2010.07.017</idno>
<idno type="wicri:Area/PubMed/Corpus">000688</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000688</idno>
<idno type="wicri:Area/PubMed/Curation">000684</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000684</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000712</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000712</idno>
<idno type="wicri:Area/Main/Merge">000134</idno>
<idno type="wicri:Area/Main/Curation">000134</idno>
<idno type="wicri:Area/Main/Exploration">000134</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars.</title>
<author><name sortKey="Daldoul, Samia" sort="Daldoul, Samia" uniqKey="Daldoul S" first="Samia" last="Daldoul">Samia Daldoul</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre de Biotechnologie de Borj cédria, Laboratoire de Physiologie Moléculaire des Plantes, B.P.901, 2050 Hammam-Lif, Tunisia; RLP-Agroscience GmbH/Alplanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt and der Weinstraße, Germany. samiabiotech@yahoo.fr</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Centre de Biotechnologie de Borj cédria, Laboratoire de Physiologie Moléculaire des Plantes, B.P.901, 2050 Hammam-Lif, Tunisia; RLP-Agroscience GmbH/Alplanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt and der Weinstraße</wicri:regionArea>
<wicri:noRegion>67435 Neustadt and der Weinstraße</wicri:noRegion>
<wicri:noRegion>67435 Neustadt and der Weinstraße</wicri:noRegion>
<wicri:noRegion>67435 Neustadt and der Weinstraße</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guillaumie, Sabine" sort="Guillaumie, Sabine" uniqKey="Guillaumie S" first="Sabine" last="Guillaumie">Sabine Guillaumie</name>
</author>
<author><name sortKey="Reustle, Gotz M" sort="Reustle, Gotz M" uniqKey="Reustle G" first="Götz M" last="Reustle">Götz M. Reustle</name>
</author>
<author><name sortKey="Krczal, Gabi" sort="Krczal, Gabi" uniqKey="Krczal G" first="Gabi" last="Krczal">Gabi Krczal</name>
</author>
<author><name sortKey="Ghorbel, Abdelwahed" sort="Ghorbel, Abdelwahed" uniqKey="Ghorbel A" first="Abdelwahed" last="Ghorbel">Abdelwahed Ghorbel</name>
</author>
<author><name sortKey="Delrot, Serge" sort="Delrot, Serge" uniqKey="Delrot S" first="Serge" last="Delrot">Serge Delrot</name>
</author>
<author><name sortKey="Mliki, Ahmed" sort="Mliki, Ahmed" uniqKey="Mliki A" first="Ahmed" last="Mliki">Ahmed Mliki</name>
</author>
<author><name sortKey="Hofer, Michael U" sort="Hofer, Michael U" uniqKey="Hofer M" first="Michael U" last="Höfer">Michael U. Höfer</name>
</author>
</analytic>
<series><title level="j">Plant science : an international journal of experimental plant biology</title>
<idno type="eISSN">1873-2259</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Salt stress adversely affects the growth of grapevine plants. In order to understand the molecular basis of salt stress response in grapevine plants, suppression subtractive hybridization (SSH) and microarray based screening approaches were combined. Two leaf-specific subtractive cDNA libraries were constructed from grapevine plants subjected to a moderate, incremental salt stress treatment. SSH were performed 6h and 24h after NaCl peaked at 100mM using cDNAs prepared from leaves of a salt tolerant cultivar (Razegui) as testers and cDNAs from unstressed leaves as drivers. Then, a pre-screened subset of cDNA clones from these SSH libraries were used to construct a Vitis vinifera cDNA array, in order to verify the expression changes of the genes upon salt treatment. Expression profiles were compared between the salt tolerant and a susceptible cultivar (Syrah) under both control conditions and after salt stress treatment. Seven cDNA clones were identified which were up-regulated by salt stress in two independent growth experiments and confirmed by RNA blot analysis. The transcript expression patterns of the selected genes differed between the contrasting grapevine cultivars tested with respect to stress-regulation. The possible relationship of individual cDNAs with salinity tolerance mechanisms is discussed.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
</list>
<tree><noCountry><name sortKey="Delrot, Serge" sort="Delrot, Serge" uniqKey="Delrot S" first="Serge" last="Delrot">Serge Delrot</name>
<name sortKey="Ghorbel, Abdelwahed" sort="Ghorbel, Abdelwahed" uniqKey="Ghorbel A" first="Abdelwahed" last="Ghorbel">Abdelwahed Ghorbel</name>
<name sortKey="Guillaumie, Sabine" sort="Guillaumie, Sabine" uniqKey="Guillaumie S" first="Sabine" last="Guillaumie">Sabine Guillaumie</name>
<name sortKey="Hofer, Michael U" sort="Hofer, Michael U" uniqKey="Hofer M" first="Michael U" last="Höfer">Michael U. Höfer</name>
<name sortKey="Krczal, Gabi" sort="Krczal, Gabi" uniqKey="Krczal G" first="Gabi" last="Krczal">Gabi Krczal</name>
<name sortKey="Mliki, Ahmed" sort="Mliki, Ahmed" uniqKey="Mliki A" first="Ahmed" last="Mliki">Ahmed Mliki</name>
<name sortKey="Reustle, Gotz M" sort="Reustle, Gotz M" uniqKey="Reustle G" first="Götz M" last="Reustle">Götz M. Reustle</name>
</noCountry>
<country name="Allemagne"><noRegion><name sortKey="Daldoul, Samia" sort="Daldoul, Samia" uniqKey="Daldoul S" first="Samia" last="Daldoul">Samia Daldoul</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000134 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000134 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= MaghrebDataLibMedV2 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:21802607 |texte= Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21802607" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a MaghrebDataLibMedV2
This area was generated with Dilib version V0.6.38. |